By Eberhard Passarge
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Additional resources for Color Atlas of Genetics (Thieme Flexibook)
On the ABI PRISM 7900HT sequence detection system instrument platform providing at least two negative ‘no template’ control (NTC) wells are included on each plate, and a user-defined quality value is set, the analysis software will attempt to objectively assign genotypes using an algorithm based on the fluorescence ratio of the dye molecules in each sample. Results are displayed on a scatter plot and cluster according to genotype, each axis represents change in 42 McBride fluorescence for a different dye fluorophore; for example, HPA-1a and HPA-1b homozygous samples will exhibit only raised FAM or VIC fluorescence, respectively, whereas heterozygotes will have intermediate fluorescence values for both dyes.
8. , Silver, J. (1997) Nanoliter scale PCR with TaqMan detection. Nucleic Acids Res 25, 1999–2004. 9. , Livak, K. , Kwok, P-Y. (2001) Fluorescence polarisation in homogeneous nucleic acid analysis II: 5 -nuclease assay. Genome Res 11, 436–440. 10. Kwok, P-Y. (2002) SNP Genotyping with fluorescence polarization detection. Human Mutation 19, 315–323. 11. Kutyavin, I. , Afonina, I. , Gorn, V. , Lukhtanov, E. , Belousov, E. , Singer, M. , Walburger, D. , Lokhov, S. , Gall, A. , Reed, M. , Meyer, R.
Transfusion 22, 70–71. , Macey, R. , Gargus, J. , Gunn, R. B. (1991) Urea transport deficiency in Jk(a-b) erythrocytes. Am J Physiol 260, C778–C783. , et al. (1997) The molecular basis of the Kidd blood group polymorphism and its lack of association with type 1 diabetes susceptibility. Hum Mol Genet 6, 1017–1020. Irshaid, N. , Olsson, M. L. (1998) Genomic typing of the Kidd blood group locus by a single-tube allele specific primer PCR technique. Br J Haematol 102, 1010–1014. 17. , et al. (2000) Molecular heterogeneity of the Jk-null phenotype: expression analysis of the Jk S291P mutation found in Finns.
Color Atlas of Genetics (Thieme Flexibook) by Eberhard Passarge