By Maryse St-Louis (auth.), Peter Bugert (eds.)
Blood samples have regularly confirmed to be a key resource of genetic fabric for a wide selection of diagnostic or learn reasons. In DNA and RNA Profiling in Human Blood: equipment and Protocols, top overseas specialists give a contribution either verified and lately constructed protocols for advanced and high-throughput DNA and RNA profiling. Divided into thorough sections, the amount concentrates on DNA profiling for blood mobilephone antigens via tools on high-throughput multiplex methods and SNP typing, besides RNA profiling in blood cells addressing sure blood telephone varieties equivalent to platelets, reticulocytes, and megakaryocytes. Written within the hugely profitable Methods in Molecular Biology™ sequence structure, the entire chapters contain short introductions at the topic, lists of the required fabrics and reagents, step by step, without difficulty reproducible laboratory protocols, in addition to the Notes part which highlights tips about troubleshooting and keeping off recognized pitfalls.
Authoritative and state of the art, DNA and RNA Profiling in Human Blood: tools and Protocols is a perfect consultant to the molecular profiling ways that experience spread out this huge box of analysis and feature proven nice promise within the additional deciding upon of sickness markers in blood.
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Additional info for DNA and RNA Profiling in Human Blood: Methods and Protocols
On the ABI PRISM 7900HT sequence detection system instrument platform providing at least two negative ‘no template’ control (NTC) wells are included on each plate, and a user-defined quality value is set, the analysis software will attempt to objectively assign genotypes using an algorithm based on the fluorescence ratio of the dye molecules in each sample. Results are displayed on a scatter plot and cluster according to genotype, each axis represents change in 42 McBride fluorescence for a different dye fluorophore; for example, HPA-1a and HPA-1b homozygous samples will exhibit only raised FAM or VIC fluorescence, respectively, whereas heterozygotes will have intermediate fluorescence values for both dyes.
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Transfusion 22, 70–71. , Macey, R. , Gargus, J. , Gunn, R. B. (1991) Urea transport deficiency in Jk(a-b) erythrocytes. Am J Physiol 260, C778–C783. , et al. (1997) The molecular basis of the Kidd blood group polymorphism and its lack of association with type 1 diabetes susceptibility. Hum Mol Genet 6, 1017–1020. Irshaid, N. , Olsson, M. L. (1998) Genomic typing of the Kidd blood group locus by a single-tube allele specific primer PCR technique. Br J Haematol 102, 1010–1014. 17. , et al. (2000) Molecular heterogeneity of the Jk-null phenotype: expression analysis of the Jk S291P mutation found in Finns.
DNA and RNA Profiling in Human Blood: Methods and Protocols by Maryse St-Louis (auth.), Peter Bugert (eds.)