By V. Mizrahi, P. A. Benkovic, R. D. Kuchta, M. C. Young, K. A. Johnson, S. J. Benkovic (auth.), P. Boon Chock, Charles Y. Huang, C. L. Tsou, Jerry H. Wang (eds.)
Recent advancements in strategies and strategies have introduced enzyme study to a altering but fascinating degree. Enzymes have served as imperative instruments within the out of the ordinary upward thrust of molecular biology, and the consequent biotechnology thrusts enzymes to new heights and territories. This quantity, the lawsuits of a up to date symposium at the Dynamics of Soluble and Immobilized Enzyme platforms, offers a present evaluate of the sector to aid scientists make the most of usual and newly received information.
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Extra info for Enzyme Dynamics and Regulation
And Landau, B. R. (1985) J. BioI. Chem. 260: 8777-8782. 18. Spence, J. , and Koudelka, A. P. (1985) J. BioI. Chem. 260: 1521-1526. 19. Seitz, H. , Muller, M. , and Tarnowski, W. (1977) Arch. Biochem. Biophys. 183: 647-663. 20. , and Buc, J. (1974) Eur. J. Biochem. 49: 195-208. 21. Ainslie, G. , Shill, J. , and Neet, K. E. (1972) J. BioI. Chem. 247:70887096. 22. Olavarria, J. , Cardenas, M. , and Niemeyer, H. (1982) Arch. BioI. Med. Exp. 15: 365-369. 23. Wells, B. , Stewart, T. , and Fisher, J.
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Although F AS is known to dissociate in low ionic strength buffer, dissociation requires hours for completion and is too slow to account for the change in reactivity. A plausible minimum model would 3. Fatty Acid Synthetase of Chicken Liver 21 100 , en ~ "j ~ E N \0 50 -'" a 100 200 Sa It Concentration (mM) FIG. 2. Dependence of second-order rate constant of inactivation on salt concentration. 0, 25°C, and salt as indicated. The salts are as follows: (0) Na phosphate, (A) KCl, and (D) Tris-Cl. (e) is rate constant obtained at 3 mM EDTA, 1% (v/v) glycerol without added salt.
Enzyme Dynamics and Regulation by V. Mizrahi, P. A. Benkovic, R. D. Kuchta, M. C. Young, K. A. Johnson, S. J. Benkovic (auth.), P. Boon Chock, Charles Y. Huang, C. L. Tsou, Jerry H. Wang (eds.)