By Chandra S. Pundir
This e-book is the 1st publication in English on nanotechnology and nanomaterials integrating with enzymatic platforms, with a spotlight on nanoparticles and organic functions. It covers comprehensively the suitable issues to appreciate the improvement of enzyme nanoparticles because it pertains to the complex constructions of enzyme nanoparticles and their functionalization and immobilization directly to a number of helps. The education of enzyme nanoparticles, their kinetic homes and functions after immobilization of the immobilized enzyme nanoparticles is defined. using color photographs in all codecs of the ebook will increase the certainty of the subjects lined. The e-book bargains an integration of Enzymology and Nanotechnology and offers the most recent details on guidance of enzyme nanoparticles, their characterization, their functionalization and immobilization directly to a variety of helps and thereafter their kinetic homes and functions in quite a few industries with unique connection with Biosensor Technology.
- Focus on enzyme nanotechnology, given the vast charm of enzymes for diagnostics, treatment and biocatalysis
- Provision of a basic heritage to the subject, but additionally an in depth description of synthesis, training and functions
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Extra info for Enzyme Nanoparticles: Preparation, Characterisation, Properties and Applications
3). 4). 3 Serum Uric Acid Levels in Apparently Healthy Individuals and Diseased Persons as Measured by Biosensor Based on Uricase NPs Modified Au Electrode S. No. 803 Source: Chauhan et al. (2014). 4 Urine Uric Acid Levels in Apparently Healthy Individuals and Diseased Persons as Measured by Biosensor Based on Uricase NPs Modified Au Electrode S. No. 68 Source: Chauhan et al. (2014). 10 Effect of uric acid concentration on response of uric acid biosensor based on uricase NPs modified Au electrode.
All rights reserved. 1 Concept of a biosensor. , 2014). Direct adsorption of proteins/enzymes onto bulk metal surfaces frequently results in denaturation of protein and loss of bioactivity. In contrast, when the enzymes are adsorbed onto metal nanoparticles, and the enzyme-covered nanoparticles are then electrodeposited onto bulk electrode surface to construct biosensors, the bioactivity of enzymes is often retained. 2). 2 Variation of current as a function of glucose concentration using modified Pt electrode ( ) Au-NPs-GOD Complex and ( ) GOD NPs.
2011). the construction, application and evaluation of amperometric/DO metric biosensors based on immobilized ENPs of horseradish peroxidase (HRP), GOD, cholesterol oxidase (ChOx) and uricase and their comparison with earlier biosensors. HORSERADISH PEROXIDASE (HRP) NPS-BASED BIOSENSOR The determination of H2O2 is very important, as it is not only the product of highly selective oxidases but also employed in various fields such as food and pharmaceutical industries and environmental analysis. 1 mmol/l H2O2 into 1 ml of stirring PB.
Enzyme Nanoparticles: Preparation, Characterisation, Properties and Applications by Chandra S. Pundir