By Surinder Kumar
Necessities of Microbiology is an in depth consultant to all features of microbiology overlaying immunology, bacteriology, virology, clinical mycology, diagnostic clinical microbiology, and plenty of miscellaneous infections. The publication is split into 89 chapters throughout seven sections. every one bankruptcy starts with an summary and concludes with key issues, a number of selection, brief and lengthy questions. bacteriology sections are integrated, the 1st overlaying the fundamentals of normal bacteriology, and the second one overlaying systemic bacteriology, with dialogue at the category, antigen constitution, pollution and enzymes, and laboratory prognosis of assorted types of micro organism. The virology part covers virus constitution, category and evolution, their interplay with host organism body structure and immunity, the ailments they reason, and their use in learn and treatment. The mycology part covers fungal infections, and among miscellaneous infections lined are microbes of the human physique, hospital-acquired infections and health center waste administration. necessities of Microbiology is more desirable by means of over 2 hundred photos and illustrations and 181 tables. the ultimate bankruptcy on functional microbiology for MBBS scholars makes this e-book perfect for scientific undergraduates. Key issues * entire consultant to microbiology * Covers immunology, bacteriology, virology, scientific mycology, diagnostic scientific microbiology, and lots of miscellaneous infections *208 photographs and illustrations, 181 tables
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Water is added on the bottom and there is a perforated shelf above water level. Articles to be sterilized are placed on the perforated shelf. Water in the bottom of the tank is heated by gas or electricity (Fig. 2). They are exposed to steam at atmospheric pressure for 90 minutes. One single exposure to steam for 90 minutes ensures complete sterilization. Tyndallization: An exposure of steam at 100°C for 20 minutes on three successive Fig. 2: Steamer 28 | Section 1: General Bacteriology days is called tyndallization or intermittent sterilization.
B. Chemical control: A Browne’s tube containing red solution changes to green when exposed to temperature of 121°C for 15 minutes in autoclave. It indicates proper sterilization. C. Thermocouples: These may also be used which record the temperature by a potentiometer. 4. g. sera, solutions of sugars or antibiotics used for the preparation of culture media. Uses 1. Heat-sensitive solutions: For sterilization of pharmaceuticals, ophthalmic solutions, culture media, oils, antibiotics and other heat-sensitive solutions.
Central, not bulging; 3. Subterminal, bulging; 4. Subterminal, not bulging; 5. Terminal, spherical; 6. Terminal, oval ii. Modified Ziehl-Neelsen (ZN) staini ng: Spores are slightly acid-fast. 5% sulfuric acid (instead of 20% sulfuric acid as used in conventional method) as decoloring agent is used for spore staining. Uses of Spores 1. Importance in food, industrial, and medical microbiology 2. Sterilization control: For proper sterilization, spores of certain species of bacteria are e mp l oye d a s i n d i cat o r, e.
Essentials of Microbiology by Surinder Kumar