Download PDF by P.F.Smith- Keary, C.J. Skidmore: Genetic Elements in Escherichia coli

By P.F.Smith- Keary, C.J. Skidmore

ISBN-10: 0333442687

ISBN-13: 9780333442685

ISBN-10: 1349194913

ISBN-13: 9781349194919

This is often written for college students of molecular genetics and molecular biology and because the first quantity during this sequence, units the scene through introducing points that offer the medical backround for the most important technical advances of genetic engineering. relevant subject matters comparable to replication, mapping, mutation, transposition and either common and site-specific recombination are totally documented. during the textual content the emphasis is at the figuring out of experimental info and every bankruptcy concludes with self-assessment questions and proposals for extra interpreting, either one of that are key positive factors of the entire books within the sequence. the writer works at one of many few departments of genetics within the united kingdom. His particular examine pursuits are bacterial genetics and the research of unitable genes.

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Extra resources for Genetic Elements in Escherichia coli

Sample text

It is thought that two different mechanisms are involved in the generation of spontaneous deletions. 8). Deletions are frequently induced as a consequence of the presence of a transposable element, when a segment of DNA immediately adjacent to the element is lost. 5) is particularly effective in deletion formation ( 10- 4 to 10- 3 cells), while among lysogens isolated following infection with the transposable bacteriophage Mu, 10-15 per cent have lost a segment of bacterial DNA adjacent to the prophage.

With this method the labour involved in mapping is greatly reduced and, because it depends only on the presence or absence of wild type transductants and not on the frequencies of different classes of transductant, the accuracy of mapping is very greatly increased. The only requirement for deletion mapping is a series of suitable deletion mutants; such mutants are available for many, but for by no means all, regions of the E. coli chromosome. Deletions arise spontaneously in certain regions of the chromosome and their occurrence seems to be associated with short, directly repeated sequences--deletion removes one of these sequences and all the DNA in between them.

Rev. , 49, 421 ( 1980). , Blackwell, Oxford ( 1986). Scott,J. , 'Regulation of plasmid replication', Microbial. , 48, 1 ( 1983). 1 Introduction Transduction was discovered by Joshua Lederberg and Norton Zinder in 1951, while looking for a system of conjugation and recombination (chapter 4) in Salmonella typhimurium. When they grew together two different mutant strains, one of which was lysogenic for phage 22 ( P22 ), they were able to recover rare prototrophs from the mixed culture. They were surprised to find that cell contact was not required since prototrophs were still reeovered when the two strains were grown side by side but separated by a fine sintered glass filter through which the bacteria could not pass, and they concluded that this genetic exchange was promoted by a DNase-resistant filterable agent.

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Genetic Elements in Escherichia coli by P.F.Smith- Keary, C.J. Skidmore


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