By Paul C. H. Li, Abootaleb Sedighi, Lin Wang
This quantity offers updates of this validated box in either tools and purposes, in addition to advances in functions of the microarray strategy to biomarkers akin to DNAs, RNAs, proteins, glycans and entire cells. Written for the Methods in Molecular Biology sequence, chapters contain introductions to their respective themes, lists of the required fabrics and reagents, step by step, simply reproducible laboratory protocols, and tips about troubleshooting and averting recognized pitfalls.
Authoritative and functional, Microarray expertise: tools and Applications goals to make sure winning leads to the extra examine of this important field.
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Additional resources for Microarray Technology: Methods and Applications
7 Scanning and Analysis 3 1. Microarray scanner GenePix 4200AL (Molecular Devices). 2. 0 software (Molecular Devices). 1 Glass Slide Preparation 1. Place five glass slides in a Coplin staining jar with 40 mL of acetone and sonicate for 10 min (see Note 3). 2. Dry glass slides under nitrogen gas for 20 min (see Note 4). 3. Place glass slides in detergent solution in a microarray highthroughput wash station and mix for 20 min (see Note 5). 4. Rinse twice with doubly distilled water in Microarray highthroughput wash station.
Piner RD, Zhu J, Xu F et al (1999) ‘Dip-pen’ nanolithography. Science 283(5402):661–663 5. Ginger DS, Zhang H, Mirkin CA (2004) The evolution of Dip-Pen nanolithography. Angewandte Chemie 43(1):30–45 6. Avseenko NV, Morozova TY, Ataullakhanov FI et al (2001) Immobilization of proteins in immunochemical microarrays fabricated by electrospray deposition. Anal Chem 73(24):6047–6052 22 C. A. Marquette 7. Nolan JP, Sklar LA (2002) Suspension array technology: evolution of the flat-array paradigm. Trends Biotechnol 20(1):9–12 8.
3. Incubate the slides for 1 h in the spotter humidity chamber (70–80 %) at room temperature (see Note 10). 4. Using the modified ODNs in the 384-well plate, perform spotting on the slides. 6 Hybridization 1. Immerse the spotted slides in the High-Throughput Wash station filled with 1 % SDS solution (500 mL) at room temperature and stir for 1 min. 2. Next, fill the station with milliQ H2O (500 mL, T = 90–100 °C) and immerse the slides in it and stir for 30 s. 3. Take out the slides and dry them by centrifugation (10 s) (see Note 11).
Microarray Technology: Methods and Applications by Paul C. H. Li, Abootaleb Sedighi, Lin Wang