By O. C. Yoder (auth.), Keisuke Kohmoto, Olen C. Yoder (eds.)
For investigators engaged within the learn of poisons typically, and host-specific pollutants specifically, it's a infrequent deal with to wait a gathering during which pollutants thinking about plant pathogenesis are emphasised. a meeting of this sort offers chance to think about the invention of latest pollutants, their chemical constructions, genes encoding enzymes that regulate their biosyntheses, their websites of motion and physiological results on crops, and their roles (if any) in pathological strategies. Having said the muse fostered by means of a 'toxin meeting', although, you will need to indicate that this system of this symposium was once generously sprinkled with 'nontoxin' talks. those contributions generated cross-disciplinary dialogue and promoted new methods of brooding about relationships between components required for plant illness improvement. the purpose will be illustrated through contemplating only one instance. now we have long ago usually looked illnesses mediated via host-specific pollutants and illnesses concerning 'gene-for-gene' relationships as representing diverse sessions of fungal/plant interplay. this can be principally as the key molecular attractiveness occasion in so-called 'toxin' illnesses results in compatibility, while the corresponding occasion in 'gene-for-gene' ailments ends up in incompatibility. but the race particular elicitors produced through the 'gene-for-gene' fungi Cladosporium fulvum (De Wit, Adv. Bot. Res. 21:147- 185, 1995) and Rhynchosporium secalis (Rohe et a1. , EMBO J.
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Additional resources for Molecular Genetics of Host-Specific Toxins in Plant Disease: Proceedings of the 3rd Tottori International Symposium on Host-Specific Toxins, Daisen, Tottori, Japan, August 24–29, 1997
Host recognition by toxigenic plant pathogen. Experientia 47:755-764. 41 11. Kuspa, A. F. (1992). Tagging developmental genes in Dictyostelium by restriction enzyme-mediated integration of plasmid DNA. Proc. Natl. Acad. Sci. USA 89:8803-8807. 12. R. C. (1994). Tagged mutations at the Toxi locus of Cochliobolus heterostrophus by restriction enzymemediated integration. Proc. Natl. Acad. Sci. USA 91:12649-12653. 13. Mills, D. and McCluskey, K. (1990). Electrophoretic karyotypes of fungi: The new cytology.
Genet. 248:547-552. 4. C. (1996). A polyketide synthase is required for fungal virulence and production of the polyketide T-toxin. Plant Cell 8:2139-2150. 5. , Kohmoto, K. and Otani, H. (1990). Determination of host-selective toxin production during spore germination of Alternaria alternata by high-performance liquid chromatography. Phytopathology 80:1088-1091. 6. N. P. (1983). o-Phthaldialdehyde precolumn derivatization and reversed-phase high-performance liquid chromatography of polypeptide hydrolysates and physiological fluids.
To isolate trichothecene resistance genes, we have screened F sporotrichioides cDNA libraries in a trichothecene-sensitive strain of S. cerevisiae. A single gene was identified in this manner and designated as TRf. Cosmids containing TRf do not overlap cosmids carrying the trichothecene pathway gene cluster which suggests this gene is not part of the gene cluster. Alignments between the TRf product and known proteins have identified a protein predicted by a yeast ORF as the only closely related sequence in the protein databases (45% identity).
Molecular Genetics of Host-Specific Toxins in Plant Disease: Proceedings of the 3rd Tottori International Symposium on Host-Specific Toxins, Daisen, Tottori, Japan, August 24–29, 1997 by O. C. Yoder (auth.), Keisuke Kohmoto, Olen C. Yoder (eds.)